Journal: Angiogenesis
Article Title: Multimodality imaging reveals angiogenic evolution in vivo during calvarial bone defect healing
doi: 10.1007/s10456-023-09899-0
Figure Lengend Snippet: Multimodality imaging system for characterizing angiogenic evolution in vivo in a preclinical calvarial defect healing model. a Photograph of the multimodal in vivo imaging system. The mouse was continuously anesthetized in a customized mouse holder connected to an isoflurane vaporizer for controlled anesthesia. The illumination source for in vivo Intrinsic Optical Signal (IOS) imaging was derived from a white light source and a filter wheel equipped with 570 ± 2 nm and 600 ± 8 nm bandpass filters. The illumination source for in vivo Laser Speckle Contrast (LSC) imaging was a 632 nm He–Ne laser coupled with a beam expander to illuminate the 3 mm cranial window field of view (FoV). The scattered light passes through a 496 nm long-pass filter and a 2 × focusing lens before being detected by a CCD image sensor. The acquired images were saved to a external hard drive for further analysis. In addition, the mouse’s vital parameters (i.e., heart rate, respiration, systemic oxygen saturation or SpO2, and body temperature) were tracked using a physiological monitor during all in vivo imaging. b Photograph illustrating the customized mouse holder, physiological monitoring sensors and heating pad employed during in vivo imaging. c Schematic illustrating a 2 mm full-thickness calvarial defect created on the murine parietal bone. A 3 mm cover slip was glued on the calvarial bone to protect the defect while permitting longitudinal in vivo imaging of angiogenesis and osteogenesis
Article Snippet: A physiological monitoring system (PhysioSuite ® , Kent Scientific Corporation, CT) was used to monitor and maintain the physiological status of the mice, including heart rate, systemic oxygen saturation (SpO 2 ), respiration rate, and body temperature.
Techniques: Imaging, In Vivo, In Vivo Imaging, Derivative Assay